As D-Lys3-GHRP-6 decreased alcohol consumption in our studies, the first concern that must be considered is whether the drug and/or the dose tested have any non-specific effects on the activity of the animals. In preliminary experiments, we tested a lower dose, 200 nmol, of D-Lys3-GHRP-6 and saw no effect on the number of ethanol-induced c-Fos cells in the pIIIu (unpublished observations) and therefore, used a higher dose in the studies detailed here. In our preference experiment (Experiment 1), we showed no difference in the total volume consumed by the saline-treated mice or the mice treated with 400 nmol of D-Lys3-GHRP-6. We can, therefore, conclude that as the animals drank similar amounts of fluid, they were equally able to access the drinking tubes. The mice treated with the antagonist consumed more water whereas the saline-treated group consumed more ethanol.
