Within each experiment for each cell type, luciferase expression levels relative to a renilla control vector were normalized to the control construct, which was set at 1.0. A one-way ANOVA comparing the mean expression of the five constructs (control, common, mixed, minor, short common, and short minor) was conducted using SPSS 16.0. Tukey’s post hoc test was used to determine statistically significant differences between individual pairs of constructs. Secondary ANOVA analysis was conducted using data from only the three long constructs and control.
