We saw a modest decrease in expression of myelin basic protein (MBP), a major component of myelin, in oligodendrocytes from individuals with AUD (FDR = 0.11). Graph-based subclustering of oligodendrocytes revealed three major subclusters, one of which had significantly lower MBP expression than the other two (Fig. 4d). This cluster was marked by high expression of OLIG2. We found 669 motifs with a significant difference in enrichment in those with AUD (FDR < 0.2), including OLIG2 (FDR = 0.04, Supplementary Data 17). A pseudobulk-level ANOVA confirmed a modest enrichment (p = 0.12, Fig. 4e). This gene was identified as a “driver” by LINGER, having higher regulatory activity in individuals with AUD based on the chromatin accessibility of its target genes.
