Illumina received a DNA manifest listing the plate number, well position, and DNA concentration determined using PicoGreen for 1,396 samples. Twenty-five of these samples were below the Illumina concentration specifications. CIDR provided a second submission for each of these low-concentration samples. Both the original and replacement DNAs were genotyped and genotypes were reported for the higher quality sample. Illumina received a revised DNA manifest document listing the plate barcode, well position, concentration, and indicating the replacement relative to the original sample. Illumina received 16 96-well DNA plates containing 1,421 samples that included 25 second aliquots. CIDR placed 92 samples per plate, leaving wells A01–A04 empty for Illumina DNA control samples. Sixteen DNA plates were accessioned into the Laboratory Information Management System (LIMS) using uniquely barcoded plates.
