The NMDA receptor antagonist D-AP5 (10 μM) had no effect on control γ power but enhanced the ethanol-induced suppression of γ (example in Figure 6A). On average, 50 mM ethanol caused a 55.1 ± 5.4% decrease in γ power in the presence of D-AP5 (P < 0.01 vs. D-AP5 baseline, n = 8), which was bigger than the effect of ethanol alone (Student t-test, P < 0.01 vs. 50 mM ethanol alone, Figure 6B). Similarly, the suppression caused by 100 mM ethanol in the presence of D-AP5 (75.7 ± 6.1% decrease, P < 0.001 vs. D-AP5 baseline, n = 8) was bigger than that in the absence of D-AP5 (Student t-test, P < 0.05 vs. 100 mM ethanol alone, Figure 6B). These results suggest that NMDA receptor blockade enhanced ethanol-induced suppression of γ oscillations.
