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Chunk #26 — Methods — Mice

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FoxO1 in dopaminergic neurons regulates energy homeostasis and targets tyrosine hydroxylase.
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All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at Wonju College of Medicine, Yonsei University. Mice were kept in controlled room temperature (22-24 °C) with a 12-h light/dark cycle (light on/off at 06:00/18:00 hours) with NC (Research Diets, Cat.No. 7001; 4.25% kcal from fat, 3.82 kcal g−1) or HFD (Research Diets, Cat.No. D12492, 60% fat, 5.24 kcal g−1).