increasing expression patterns, respectively, across regions R1, R3, R5, and R6 (Fig. 2d). Second, we used RNA-sequencing (RNA-seq) data from 88–129 individuals in the GTEx consortium19 and selected samples of the substantia nigra (R3), amygdala (R4), anterior cingulate cortex (R5), and frontal cortex (R6). For the 883 BRGs present in the GTEx consortium, 204 out of 318 (64.2%) negatively correlated BRGs and 475 out of 565 (84.1%) positively correlated BRGs were differentially expressed between the two most distant regions R3 and R6 in this dataset (FCR3–R6| > 1, BH-corrected P < 0.05, DESeq2). The mean expression of BRGs again showed decreasing and increasing patterns, here across regions R3-R6 (Fig. 2e). Together, this indicates that the expression patterns of BRGs in the brain are consistent across non-neurological individuals.
