Chunk #23 — Results — Snail mRNA expression is significantly elevated in the colonic mucosa of heavy drinkers

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Alcohol stimulates activation of Snail, epidermal growth factor receptor signaling, and biomarkers of epithelial-mesenchymal transition in colon and breast cancer cells.

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To investigate the potential relevance of our in vitro findings in vivo, we sought to determine Snail mRNA expression in the colonic mucosa of chronic (active) heavy alcohol drinkers. We used qRT-PCR to assess Snail mRNA expression using RNA prepared from intestinal biopsies from normal vs. alcoholic subjects. As shown in Figure 4D, Snail mRNA expression was significantly higher (83% higher) in colonic biopsies from chronic heavy drinkers (NIAAA criteria) as compared to controls (N=4/group, p≤.05, t-test).