dpi (Figure 4A). To control for Ca2+ dye loading efficacy, we normalized all responses to those of Ca2+ ionophore ionomycin (10 µM) for each cell. No significant decrease in the peak amplitude of muscimol-induced Ca2+ responses was observed up to 11 dpi (Figure 4B). Furthermore, muscimol-induced Ca2+ rise was absent in shRNA-NK1+ neurons at 7 dpi (Figures 4A to 4B). These results provide additional evidence that GABA depolarizes newborn neurons up to 14 dpi in an NKCC1-dependent fashion during adult hippocampal neurogenesis.
