In the case of the tumor suppressor gene PTEN, we find an almost identical duplicate (97% coverage, 98% identity) corresponding to PTENP1 (Figure 2A). While the activity of PTEN as repressor of the AKT pathway is well documented (42,43), PTENP1 is known to transcribe a processed pseudogene (44,45) but the involvement in cancer has never been reported. At 10% coverage, an additional hit is found, which involves the last 50 amino acids of PTEN and matches to the intronic region between exons 3 and 4 of ANKFN1 (ankyrin-repeat and fibronectin type III domain containing 1, Figure 2A).
