Chunk #13 — MATERIALS AND METHODS — Control experiments of electron microscopic immunocytochemistry

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Endogenous GluR1-containing AMPA receptors translocate to asymmetric synapses in the lateral amygdala during the early phase of fear memory formation: an electron microscopic immunocytochemical study.

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Two types of control experiments were conducted. One was the “no-primary-antibody” control, in which a subset of grids holding adjacent or nearly adjacent ultra-thin sections to those that were processed for GluR1 or GluR2/3 immunocytochemistry was incubated overnight in the identical TBST buffer (pH 7.4; NaCl concentration at 0.3%) but containing no primary antibody. Tissue from every animal underwent this control, every time the PEG experiment was run.