Ethanol increases GABAergic transmission in CeA with a maximally effective concentration of 44 mM (Roberto et al., 2003). We first confirmed these results and found that superfusion of 44 mM ethanol significantly increased IPSP amplitude to 148 ± 5% of control (n = 8, t = 8.159; Fig. 3A). PPF analysis indicated that ethanol decreased the paired-pulse ratio to 66 ± 6% of control (n = 8; not shown), confirming a presynaptic site of action for ethanol-induced increase in GABAergic transmission.
