As the SNP in PRKCH (rs2230500) underlying the previous association in Japanese cohorts1 is monomorphic in populations of European ancestry, we sought to identify any associations within this gene region, including the 50 kbp window upstream and downstream, in our large population of European ancestry. Using the modified Nyholt correction approach of Li and Ji on the 353 SNPs from the region, we estimated the effective number of SNPs tested to be 103·3.23 We therefore set the significance level at p<0·00048, corresponding to Bonferroni corrected type I error <5% for the effective SNPs tested.
