homopolymers which overlapped the last base of the key and the first base/s of the read, we only consider base positions 10 and greater for analysis. Furthermore, analysis was restricted to the first 100 bp for the Ion OneTouch Template Kit (100 bp reads), and 200 bp for the Ion Xpress Template 200 and Ion OneTouch 200 Template kits, as the number of reads longer than this decreased rapidly leading to exaggerated error rates at these base positions.
