The replication human brain proteomes were profiled from the dPFC of post-mortem brain samples donated by 198 European participants of the Banner Sun Health Research Institute (Banner). Most subjects were enrolled as cognitively unimpaired volunteers from the retirement communities of the greater Phoenix, Arizona, USA. Subjects received standardized general medical, neurological, and neuropsychological tests annually during life. A final clinicopathological diagnosis of normal cognition or Alzheimer’s disease or other dementias was rendered after review of all standardized clinical data, the most recent medical records, and neuropathological examination findings23. All enrolled subjects or their legal representatives signed an informed consent and the study was approved by the Banner Sun Health Research Institute Institutional Review Board. Only subjects with a final clinical diagnosis of normal cognition or AD were included in the proteomic analysis. Proteomic profiling was performed using the same approach as described for the discovery proteomes with two differences: only MS2 scans were obtained and MS2 spectra were searched against the UniProtKB human brain proteome database downloaded in April 2015. A total of 11,518 proteins were quantified. We used the
