stereotaxic injections of lentiviruses encoding two independent shRNAs against Nurr1 (shNurr1-1 and shNurr1-2) or control shRNA (shCtrl) into the SN of adult wild-type mice (Fig S2A). shNurr1-1 and -2 efficiently and specifically reduced Nurr1 mRNA and protein expression in the SN, as determined by qPCR and immunostaining (Fig. S2B–E). A comparison of lentivirus-directed GFP expression with cell-specific markers indicated preferential transduction of non-neuronal cells, including microglia and astrocytes (Fig. S2G). The lentiviral injection was followed two days later by injection of LPS into the same coordinates. We then analyzed the magnitude of the inflammatory response by qPCR 6h after LPS injection and quantified TH+ neurons by immunohistochemistry (IHC) 7 days after LPS injection.
