Fibroblast cell lines GM08330 (unaffected, father), GM08329 (unaffected, mother), GM05225 (BD Type I, proband), GM05224 (BD Type I, brother) were obtained from the Coriell Cell Repository. Records showed punch biopsies for GM05224 and GM05225 were collected from the posterior iliac crest as were the two parents GM08330 and GM08329 (Dr. Elliot Gershon, personal communication). Induced pluripotent stem cells (iPSCs) were derived using individual pseudotyped retroviruses expressing MYC (MSCV-h-c-MYC-IRES-GFP, Addgene# 18119), KLF4 (pMIG-hKLF4, Addgene# 17227), SOX2 (pMIG-hSOX2, Addgene# 17226), and OCT4 (pMIG-hOCT4, Addgene#17225) packaged by Harvard Gene Therapy Core (Harvard Medical School) following methods described in29. Fibroblasts were infected with all four viruses as described30 and split onto irradiated MEFs (GlobalStem, GSC-6301G). Colonies were picked and expanded in hES media on MEFs or in mTeSR1 on Matrigel. hES media consisted of 80% DMEM/F12 (Invitrogen, 11320033), 20% Knock-out Serum Replacement (KOSR) (Invitrogen, 10828-028), 10 ng/mL bFGF (R&D systems: Cat# 233-FB-001MG/CF), 1 mM L-glutamine (Invitrogen, 35050-061), 100 µM non-essential amino acids (Gibco, 11140-050), 100 µM 2-mercaptoethanol (Sigma, M6250), 50 U/mL penicillin 50 mg/mL streptomycin (Invitrogen, 15140-122), mTeSR1 (Stemcell Technologies, 05850).
