All mice used for the mapping experiments were male and were obtained from The Jackson Laboratory (Bar Harbor, MA) at 11–12 weeks of age, and were kept in our facility a minimum of 1 week prior to use in experiments. The identity of each of the inbred strains used and the Jackson Laboratories catalog number are listed in Additional file 3: Table S1. Mice heterozygous for the deleted in colorectal carcinoma (Dcc) gene and wild-type littermates were bred from animals initially supplied by S. Ackerman (The Jackson Laboratory) [48]. The original Dcctm1Wbg mice [30] have been crossed back to C57BL6/J for a minimum of 36 generations. Cryopreserved material of this mouse strain is available at the Mutant Mouse Regional Resource Centers (https://www.mmrrc.org/catalog/StrainCatalogSearchForm.php?search_query=030626).
