The WikiGenes (https://www.wikigenes.org/) database was used to identify pathways in which these genes are involved. Gene pathway analysis for the whole gene set resulted in 75 significant pathways (adj P<0.05, S3 Table). Among the top 20 pathways, most are involved in immune system function, including multiple IL-Signaling Pathways, B Cell Receptor Signaling Pathway, T Cell Receptor Signaling Pathway, the chemokine signaling pathway, MAPK signaling pathway, toll-like receptor signaling pathways, etc. (Table 4). Pathway analysis for the ethanol treated genes resulted in 3 significant pathways: macrophage markers, MAPK signaling pathway, and G protein signaling pathways, all of which are among the top 20 pathways from the whole gene set.
