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Chunk #12 — Materials and methods — Immunohistochemistry for electron microscopy

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Developmental regulation of G protein-gated inwardly-rectifying K+ (GIRK/Kir3) channel subunits in the brain.
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Immunohistochemical reactions for electron microscopy were carried out using the immunogold methods described previously (Luján et al., 1996). Ultrastructural analyses were performed in a Jeol-1010 electron microscope.