The MAGMA 14 package was used to assess the aggregated genetic effects from the meta-analysis on 17,842 genes. This technique identified a total of 269 putative genes that were associated (P < 2.80 × 10-6) with depression, Supplementary Table 9. The most significant gene (P = 2.27 × 10-19) was Sortilin related VPS10 domain containing receptor 3 (SORCS3) on chromosome 10 (Supplementary Figure 10). The Neuronal Growth Regulator 1 (NEGR1) gene was associated with depression (P = 3.55 × 10-15) and there were two nearby independently-associated variants (rs2568958 and rs10890020) located in separate loci (Supplementary Figure 11 A-B). rs10890020 overlapped with the Long Intergenic Non-Protein Coding RNA 1360 (LINC01306) coding region which was not available for analysis in MAGMA. A further short (1.2 Mb) region along 18q.21.2 contained three independently-associated variants across two loci (rs62091461, rs12966052 and rs12967143; Supplementary Figure 12 A-C). These variants were close to the Transcription Factor 4 (TCF4) and the RAB27B genes which were both putatively associated with depression (P = 4.55 × 10-16 and P = 1.39 × 10-9, respectively). Further consideration of the genes putatively associated with depression is provided in the discussion.
