one (left) for reverse strand transcription and one (right) for forward strand transcription, in a divergent manner. The merged bidirectional pairs were further required to be bidirectionally transcribed (CAGE tags supporting both windows flanking the center) in at least one individual sample, and to have a greater aggregate of reverse CAGE tags (over all FANTOM5 samples) than forward CAGE tags in the 200 bp region associated with reverse strand transcription, and vice versa. These filtering steps resulted in 78,555 bidirectionally transcribed loci.
