Total RNA was extracted from differentiating hESC/hiPSC lines using the TRIzol Reagent (Life Tech), and 0.51 ug of RNA was reverse transcribed by High Capacity cDNA Reverse Transcription Kit RT2 first strand kit (ABIQiagen). Primer sequences are provided below. qRT-PCR mixtures were prepared with SYBR Green PCR Master Mix Universal (Applied BiosystemsKapabiosystem) and reactions were done with the Eppendorf Realplex2.
