Cnih2fl/fl and Cnih3fl/fl mice were generated by standard procedures (Figure S1). Cnih2fl/fl and Cnih3fl/fl mice were first bred as homozygotes and then Cnih2fl/fl mice were bred with Cnih3fl/fl mice and NEX-CRE mice. Importantly, homozygous Cnih2fl/fl and Cnih3fl/fl were indistinguishable from wild-type mice. In addition the NEX-CRE Cnih2fl/fl (NexCnih2−/−) mice, in which CNIH-2 is deleted from all forebrain pyramidal neurons, appeared grossly normal and breeding was Mendelian.
