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Chunk #19 — Materials and Methods — Electrophysiology

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Pilot study of iPS-derived neural cells to examine biologic effects of alcohol on human neurons in vitro.
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To observe the effects of acute alcohol exposure on GABAA and AMPA responses, the same paradigm was used as described above, except the solution being puffed onto the cell was either 50 µM GABA or 50 µM glutamate to elicit GABAA or AMPA responses, respectively. AMPA responses were observed using an external solution containing 1mM Mg2+ without the addition of DNQX.