To verify the role of CtBP2 on EMT in HCC, we used CtBP2 targeted siRNAs to silence CtBP2 expression in MHCC97H cells (Figure 4A). Consistent with the results in the Huh7 cell experiments, CtBP2 knockdown suppressed the migration and invasion of MHCC97H cells (Figure 4B and 4C). Additionally, we observed that CtBP2 suppression lead to E-cadherin upregulation in MHCC97 cells, but decreased the expression of N-cadherin, Vimentin and Fibronectin (Figure 4D). Taken together, these data strongly support the hypothesis that CtBP2 promotes HCC cell mobility and invasion by inducing EMT.
