with the average line producing up to 70%, 84% and 77% of cells expressing all three markers of dEN, dME and dEC, respectively (Fig. 1d). We assessed correlations of differentiation capacity between different germ layers and found a positive correlation between endoderm and mesoderm marker expression (Spearman r = 0.36, P < 0.001), and between endoderm and pluripotency marker expression (Spearman r = 0.21, P < 0.008) (Extended Data Fig. 1c). Taken together, our data indicate that virtually all of the iPSC lines we have derived are pluripotent, although we observed some variability in differentiation between lines.
