Chunk #67 — Materials and methods — Sequencing and analysis using Illumina MiSeq

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Detection of low prevalence somatic mutations in solid tumors with ultra-deep targeted sequencing.

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The four calibration sample microdroplet PCR libraries (CAL-A, CAL-B, CAL-C, CAL-D) were amplified using an additional six cycles of universal PCR in the same conditions described above, with the use of a reverse primer containing DNA index (CAAGCAGAAGACGGCATACGAGATXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGAC, where X indicates the Illumina index sequence), as recommended by Illumina.