marked by high expression of genes involved in endocytosis and phagocytosis. In individuals with at least 50 microglia cells, there was a significantly higher proportion of Inflammatory Microglia (subcluster 2) in individuals with AUD: 31%, versus 23% in those without AUD (false discovery rate (FDR) = 0.027). Individuals with at least half of their microglia in the inflammatory state were more likely to have AUD (70%) than those with fewer than half (52%) (Fig. 1c). There was also a significantly higher proportion in cluster 2 with increasing age (FDR = 0.029).
