through the generation of isogenic wild-type and variant hPSCs via genome editing. Consistent with prior studies, we found the generation of knock-in clones via HDR to be very inefficient compared to the generation of clones with defined deletions via multiplexed NHEJ. Nonetheless, we were able to generate knock-in clones for all three SNPs, and the gene expression changes observed in knock-in clones were concordant with those observed from SNP-deleted clones.
