indicating that here, PU.1 binding is not sufficient to establish this mark. Increased recruitment of C/EBPβ to Groups I (37%) and II (30%) relative to Group III (11%) peaks (<100 bp) suggests that additional factors may be required for deposition of H3K4me1. Group I and II peaks, but not Group III peaks, were enriched for the 464 vicinal genes that were up-regulated greater than 4-fold in PUER cells following 24 h tamoxifen treatment (Weigelt et al., 2009) (Figure S4B).