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Chunk #22 — PU.1 induces sequential nucleosome remodelling and histone H3K4 monomethylation

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Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities.
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indicating that here, PU.1 binding is not sufficient to establish this mark. Increased recruitment of C/EBPβ to Groups I (37%) and II (30%) relative to Group III (11%) peaks (<100 bp) suggests that additional factors may be required for deposition of H3K4me1. Group I and II peaks, but not Group III peaks, were enriched for the 464 vicinal genes that were up-regulated greater than 4-fold in PUER cells following 24 h tamoxifen treatment (Weigelt et al., 2009) (Figure S4B).