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Chunk #7 — Methods — Brain Volume Data

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Shared Predisposition in the Association Between Cannabis Use and Subcortical Brain Structure.
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High-resolution (0.7mm isotropic voxels) anatomical images were acquired using a customized Siemens Skyra 3T scanner with a 32-channel head coil. For details on data acquisition and preprocessing, see15 (see eMethods S2 for relevant pre-processing steps). Volume estimates for the regions of interest were extracted using FreeSurfer v5.3.016,17 (http://surfer.nmr.mgh.harvard.edu/). This included WBV (total gray + cortical white matter volume) and left and right amygdala, hippocampus, and VS volumes from subcortical segmentation and OFC volumes (lateral + medial) from cortical parcellation using the Desikan atlas18.