respectively. All calibration concentrations were calculated to be within 15% of their expected values, except the lower limit of quantification which was within 20 % of its expected concentrations. The lower limit of quantification also had a response at least five times greater than the signal to noise ratio for all sample batch. Lower limit of detection samples were analyzed and verified to have a signal to noise ratio was three times the noise level of the background signal. The linear regression correlation coefficients (r2) for the anandamide and 2-AG calibration curves were 0.990 or better for all calibration curves.
