Eight transcripts (seven genes) were found to be differentially expressed in response to trained treatment as compared to odors-only controls (Figure 3C, Figure S7). Three of these transcripts were downregulated: the Sterol Regulatory Element Binding Protein SREBP (HLH106-RC), the Drosophila homolog of dynamin shibire (shi-RP), and a predicted Ras guanine-nucleotide exchange factor CG9098-RA. Four transcripts were upregulated: [a retinoic acid-like protein CG3558-RB, the same predicted Ras guanine-nucleotide exchange factor CG9098-RB, a Coat Protein Complex II factor Secretory 31 (Sec31-RA), and a transmembrane heparin sulfate proteoglycan Syndecan (Sdc-RK)], as well as a transcription factor that functions in the JAK/STAT pathway, Signal-transducer and activator of transcription protein at 92E (Stat92E-RH). Using the DRSC Integrative Ortholog Prediction Tool (DIOPT) (Hu et al. 2011), we identified, when possible, the human genes corresponding to the implicated fly genes (Figure 3Aiii, Figure 3Biii, Figure 3Ciii).
