#2266) and β-Actin (Sigma #A5441) in 3% non-fat dry milk in TBS/0.1% Tween-20 buffer. Secondary antibody staining was visualized using WesternBright ECL HRP Substrate Kit (Advansta K-12045) and ChemiDoc XRS+ (BioRad). Images were quantified using ImageJ (NIH) and GraphPad Prism 7 (GraphPad Software). Two independent experiments were performed without randomization of sample processing, n = 2. Researcher was not blinded to the samples identification. Differences between every group mean were analyzed with one-way ANOVA and Sidak’s post hoc multiple variance test between selected groups, with a single pooled variance. Adjusted P values for each comparison are reported.
