2, 4, and 6, the Mice received saline injections and were placed in the alternative compartment after 25 min. On Day 7, mice were placed in the middle neutral compartment of the place preference apparatus with full access to the other two compartments and assessed for the length of time spent in each compartment (postconditioning report). The percentage of time spent in each association compartment was calculated relative to the total time spent in both association compartments.