As shown in Fig 2A, alcohol consumption caused the formation of lipid droplets, which were mainly distributed around the central vein in cyp2a5+/+ mice, while in cyp2a5−/− mice were also observed around portal veins except for the central veins; the size of the lipid droplets was smaller in cyp2a5+/+ mice than cyp2a5−/− mice. Also, we found more hepatocytes containing lipid droplets in cyp2a5−/− mice than cyp2a5+/+ mice (Fig. 2B white bars). Likewise, TG levels in liver and serum were increased in cyp2a5−/− mice to a greater extent than in cyp2a5+/+ mice (Fig 3A and B white bars). In addition, serum glucose was increased in cyp2a5−/− mice but not in cyp2a5+/+ mice (Fig 3B black bars). These results suggest that CYP2A5 protects against alcohol-induced fatty liver.
