We next used ABSOLUTE to convert the allelic-fraction of mutations to cellular multiplicities. For this purpose, we examined 29,268 somatic mutations identified in whole-exome hybrid capture Illumina sequencing36 data from 214 ovarian carcinoma tumor-normal pairs33 (Online Methods, Fig. 4a). Tumor purity, ploidy, and absolute copy-number values were obtained from Affymetrix SNP6.0 hybridization data on the same DNA aliquot that was sequenced, allowing the rescaling of allelic-fractions to units of multiplicity (Fig. 4a,b; Online Methods, Eq. 12).
