d, then 9% (v/v) ethanol vs. water for 2 d, then 12% (v/v) ethanol vs. water for 2 d, 15% (v/v) ethanol vs. water for 2 d, 18% (v/v) ethanol vs. water for 2 d, and finally 21% (for female mice, not for male mice). Throughout the experiment, evaporation/spillage estimates were calculated every day from two bottles placed in an empty cage, one containing water and the other containing the appropriate ethanol solution. In another experiment, mice were injected with LPS, and drinking was tested beginning 30 days after LPS administration. In this study, mice were housed singly beginning 3 d before LPS injection and throughout the remainder of the experiment.
