Chunk #55 — Online Methods — Sensitivity Calculation

Source

Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples.

Embedded

yes

Text

To calculate the sensitivity to detect a mutation with allelic fraction f using n reads having a Phred-like quality score q (and hence a base error, e, of 10-q10), we first calculate k, the minimum number of reads with the alternate allele that will trigger a variant call using