Chunk #43 — MATERIALS — REAGENT SETUP — Sequencing adapter PCR primers

Source

Detecting ultralow-frequency mutations by Duplex Sequencing.

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Dissolve each respective PCR primer in TE or ddH2O in separate microcentrifuge tubes to a final concentration of 20 μM each. Immediately freeze the oligonucleotides at −20 °C until further use. Primers can be stored at this temperature for 6–12 months.