Furthermore, we found that neural-related genes tended to be differentially methylated between Bl-iPSC and NP-iPSC (Supplementary Fig. 3b). Treatment of NP-iPSC with TSA and AZA enhances blood-forming potential and increases hypomethylated DMRs (626; Supplementary Table 1c). We found significant overlap between these DMRs and genes enriched in mouse hematopoietic stem cells (MSigDG signature STEMCELL_HEMATOPOIETIC_UP; Supplementary Fig. 3c), suggesting that drug treatment erases inhibitory methylation signatures at hematopoietic loci.
