In order to evaluate changes in Kalirin isoform expression, PSDs were prepared from the striata of mice treated chronically with saline or cocaine (Figure 9A). Analysis of equal amounts of input (S1) and PSD protein revealed increased levels of Kal7 protein in both samples. DARPP32, a soluble protein enriched in striatal neurons, was excluded from the purified PSDs, attesting to their purity. Small amounts of Kal9 and Kal12 could be detected in the purified PSDs, but Kal12 was barely detectable in the input samples. The identification of the Kal7 band was verified with the Kal7-specific antibody (Figure 9B).
