ALDH3B1 is duplicated in cow and zebra finch, as well as in zebrafish, on the proviso that D. rerio aldh3d1 is also considered an ALDH3B1 homologue. Zebrafish Aldh3d1 shares 44 per cent AA identity with Aldh3b1 and is listed in NCBI HomoloGene as a homologue of ALDH3B1 (HomoloGene, data not shown) [12]. Zebra finch ALDH3B5 encodes a 341-AA peptide that shares 100 per cent sequence identity with the 228 amino-terminal AAs of the parent gene's protein. Cow and zebra finch ALDH3B4 and ALDH3B5 proteins share 80.9 per cent and 53.2 per cent sequence identity with their respective parent genes, and 39.7 per cent with one another, indicating that none of the genes is an orthologue. Zebra finch ALDH3B5 is shorter than ALDH3B1 (341 versus 450 AAs) and, without this sequence gap, they share 93.1 per cent AA identity; it is unknown whether this smaller gene product is functional.
