S3C). Consistent with previous findings, Northern blotting of adult tissue RNA showed that both wild-type Bptf and the Bptf–β-Geo fusion alleles are specifically expressed at high levels in the testis and at moderate levels in the lung, spleen, and brain (Figure S3D) [25]. These identical RNA expression patterns initially suggest that β-galactosidase is a faithful reporter of Bptf expression in adult tissues. These results indicate that the BptfXG023 mutation creates a truncated Bptf–β-Geo fusion carrying the N-terminal 1978 residues of the 2903-residue Bptf open reading frame, which eliminates the conserved glutamine rich region, PHD finger, and bromo-domains.
