We compared the magnitude and direction of chromatin accessibility differences to the gene expression differences in genes that had at least 1 DAR in the promoter region, a total of 4,915 genes across all cell types. The AUD-associated DARs and DEGs were in the same direction for most genes in the four largest cell clusters (88%, 90%, 73%, and 77% in oligodendrocytes, astrocytes, D1 neurons, and D2 neurons, respectively), and genes containing DARs were enriched among DEGs in the same four cell types (padj < 1e-8; Fig. 4D-G). These results together suggest that AUD-associated differences in chromatin accessibility can potentially lead to a corresponding change in cis-gene expression.
