of double-labeled cells in the ventral tegmental area while nicotine pretreatment did not (Hendrickson et al. 2009). In addition, one study has shown that the CRF1 antagonist CP-154,526 reduced ethanol intake and BEC during 4 h DID sessions that resulted in relatively high intake and BECs, but was without effect in shorter sessions leading to lower intakes/BECs (Sparta et al. 2008).