After 10-weeks of HFD-feeding, IR and GT tests were evaluated in mice fasted for 6 hours and subsequently injected with insulin (0.75 U/kg; Eli Lilly) or glucose (2 g/kg). GT tests were performed 3 days after the IR test. Tail blood was collected at indicated times immediately after intraperitoneal injection of insulin (IR) or glucose (GT). Blood glucose levels were determined using the Elite glucometer (Bayer).
