changes in NOX/XOX mRNA levels by real-time PCR in RNA extracts from primary neurons treated with EtOH/Ach in the presence or absence of Chx or ACD for 48 h. EtOH/Ach exposure increased the NOX mRNA levels by 47 or 51%, respectively, compared with control, and ACD significantly inhibited this Ach-mediated increase in NOX mRNA levels by 25% (Fig. 5A). Similarly,treatment of neurons with EtOH/Ach up-regulated XOX mRNA level by 292 or 463%, respectively, compared with control (Fig. 5B), and ACD inhibited 66% of the Ach-mediated increase in XOX mRNA levels. These results suggest that the transcription process modulated the EtOH/Ach-induced increases in NOX/XOX activity and protein contents in neurons.
