We first asked whether SCZ hGPCs differed from wild-type hGPCs in their myelination competence. To this end, we implanted SCZ hGPCs into neonatal immunodeficient shiverer mice (rag2−/− × MBPshi/shi), a congenitally hypomyelinated mutant lacking myelin basic protein (MBP)(Roach et al., 1983; Rosenbluth, 1980). As these otherwise myelin-deficient mice matured, their engrafted hGPCs differentiated into both astrocytes and myelinogenic oligodendrocytes, yielding mice chimeric for individual patient-derived glia(Windrem et al., 2008; Windrem et al., 2014). By this means, we established mice with patient-specific, largely humanized forebrain white matter, derived from SCZ or control subjects (Figures 2A–D).
